The Impact of Site-Specific Light Chain Conjugation on Antibody Structure Using Hydrogen Exchange-Mass Spectrometry (HX-MS)
- Differential HX-MS was used to explore the impact of drug conjugation on the conformational stability and structural dynamics of a mAb
- A side-by-side comparison between a mAb conjugated with a pyrrolobenzodiazepine drug and an unconjuagated mAb revealed subtle conformational changes induced by the linker-drug particularly near the site of conjugation
- To gain a comprehensive understanding of these effects other complementary biophysical techniques (DSC, DSF, SEC, and icIEF) were integrated into the study